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Stromal cells from the adipose tissue-derived stromal vascular fraction and culture expanded adipose tissue-derived stromal/stem cells: a joint statement of the International Federation for Adipose Therapeutics (IFATS) and Science and the International Society for Cellular Therapy (ISCT)

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Cytotherapy 2013 Jun;15(6):641-8. doi: 10.1016/j.jcyt.2013.02.006. Epub 2013 Apr 6.

 

Stromal cells from the adipose tissue-derived stromal vascular fraction and culture expanded adipose tissue-derived stromal/stem cells: a joint statement of the International Federation for Adipose Therapeutics (IFATS) and Science and the International Society for Cellular Therapy (ISCT)
1CSA21, Toulouse, France
2Center for Stem Cell Research and Regenerative Medicine, Department of Pharmacology, Tulane University School of Medicine, New Orleans, Louisiana, USA
3StromaLab, UMR UPS/CNRS/EFS 5273, Inserm U1031, Toulouse, France
4Laboratory of Cell Biology and Advanced Cancer Therapies, Department of Medical and Surgical Sciences for Adults and Children, University Hospital of Modena and Reggio Emilia, Modena, Italy
5Division of Plastic and Reconstructive Surgery, Department of Surgery, University of Florida, Gainesville, Florida, USA
6Indiana Center for Vascular Biology and Medicine, Indiana University School of Medicine, Indianapolis, Indiana, USA
7Ludwig Boltzmann Institute for Experimental and Clinical Traumatology in AUVA Research Center, Austrian Cluster for Tissue Regeneration, Vienna, Austria
8Department of Plastic Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
9Department of Plastic Surgery, University of Tokyo School of Medicine, Tokyo, Japan
10Stem Cell Biology Laboratory, Pennington Biomedical Research Center, Louisiana State University System, Baton Rouge, Louisiana, USA
Correspondence: Dr. Philippe Bourin, CSA21, 7 chemin des silos, 31100 Toulouse, France.

 

ABSTRACT

Background aims

Adipose tissue is a rich and very convenient source of cells for regenerative medicine therapeutic approaches. However, a characterization of the population of adipose-derived stromal and stem cells (ASCs) with the greatest therapeutic potential remains unclear. Under the authority of International Federation of Adipose Therapeutics and International Society for Cellular Therapy, this paper sets out to establish minimal definitions of stromal cells both as uncultured stromal vascular fraction (SVF) and as an adherent stromal/stem cells population.

Methods

Phenotypic and functional criteria for the identification of adipose-derived cells were drawn from the literature.

Results

In the SVF, cells are identified phenotypically by the following markers: CD45-CD235a-CD31-CD34+. Added value may be provided by both a viability marker and the following surface antigens: CD13, CD73, CD90 and CD105. The fibroblastoid colony-forming unit assay permits the evaluation of progenitor frequency in the SVF population. In culture, ASCs retain markers in common with other mesenchymal stromal/stem cells (MSCs), including CD90, CD73, CD105, and CD44 and remain negative for CD45 and CD31. They can be distinguished from bone-marrow-derived MSCs by their positivity for CD36 and negativity for CD106. The CFU-F assay is recommended to calculate population doublings capacity of ASCs. The adipocytic, chondroblastic and osteoblastic differentiation assays serve to complete the cell identification and potency assessment in conjunction with a quantitative evaluation of the differentiation either biochemically or by reverse transcription polymerase chain reaction.

Conclusions

The goal of this paper is to provide initial guidance for the scientific community working with adipose-derived cells and to facilitate development of international standards based on reproducible parameters.

PMID: 23570660 PMCID: PMC3979435 

To read the full article, click here DOI: 10.1016/j.jcyt.2013.02.006

Related: adipose-derived stromal/stem cells, adipose tissue, characterization, function, phenotype, stromal vascular fraction, stem cells population